Within-Host and Population Transmission of blaOXA-48 in K. pneumoniae and E. coli

نویسندگان

  • Manon R. Haverkate
  • Mirjam J. D. Dautzenberg
  • Tjaco J. M. Ossewaarde
  • Anneke van der Zee
  • Jan G. den Hollander
  • Annet Troelstra
  • Marc J. M. Bonten
  • Martin C. J. Bootsma
  • Alex Friedrich
چکیده

During a large hospital outbreak of OXA-48 producing bacteria, most K. pneumoniaeOXA-48 isolates were phenotypically resistant to meropenem or imipenem, whereas most E. coliOXA-48 isolates were phenotypically susceptible to these antibiotics. In the absence of molecular gene-detection E. coliOXA-48 could remain undetected, facilitating cross-transmission and horizontal gene transfer of blaOXA-48. Based on 868 longitudinal molecular microbiological screening results from patients carrying K. pneumoniaeOXA-48 (n = 24), E. coliOXA-48 (n = 17), or both (n = 40) and mathematical modelling we determined mean durations of colonisation (278 and 225 days for K. pneumoniaeOXA-48 and E. coliOXA-48, respectively), and horizontal gene transfer rates (0.0091/day from K. pneumoniae to E. coli and 0.0015/day vice versa). Based on these findings the maximum effect of horizontal gene transfer of blaOXA-48 originating from E. coliOXA-48 on the basic reproduction number (R0) is 1.9%, and it is, therefore, unlikely that phenotypically susceptible E. coliOXA-48 will contribute significantly to the spread of blaOXA-48.

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عنوان ژورنال:

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2015